Antibacterial activity of Ricinus communis plant extract against antibiotic resistant Helicobacter pylori and Gluconobacter oxydans isolated from fresh apple juices samples

Abstract Bacteria were isolated from samples of Fresh Apple juices from shops of three different localities of Lahore. Analysis of samples from Liberty, Anarkali and Yateem khana Markets show different levels of contamination. There were pathogenic and non-pathogenic bacteria in all samples and were identified by the morphological and biochemical tests. Most of the plasmids of pathogenic bacteria were 4kb in their molecular size. Ribotyping of 16S ribosomal RNA gene sequencing was done to confirm Helicobacter pylori strain and Gluconobacter oxydans. The highest sensitivity of 210mm was shown by Enterobacter sp. against Aztheromysine disk (15µg) while Micrococcus sp. was highly resistant against all of the Antibiotics applied. The antibiotic resistance of pathogenic bacteria was also checked against Ricinus communis plant's extracts, all isolated bacterial pathogens were resistant but only, E.coli was inhibited at 300µl of the extracts. Presence of pathogenic bacteria in Apple juice samples was due to contamination of sewage water in drinking water while some of these pathogenic bacteria came from Apple's tree and other from store houses of fruits.

Resumo As bactérias foram isoladas de amostras de suco de maçã fresco de lojas de três diferentes localidades de Lahore. A análise de amostras dos mercados Liberty, Anarkali e Yateem khana mostram diferentes níveis de contaminação. Havia bactérias patogênicas e não patogênicas em todas as amostras e foram identificadas pelos testes morfológicos e bioquímicos. A maioria dos plasmídeos de bactérias patogênicas tinha 4 kb em seu tamanho molecular. A ribotipagem do sequenciamento do gene do RNA ribossômico 16S foi realizada para confirmar a cepa de Helicobacter pylori e Gluconobacter oxydans. A maior sensibilidade de 210 mm foi mostrada por Enterobacter sp. contra disco de azteromisina (15µg) enquanto Micrococcus sp. foi altamente resistente a todos os antibióticos aplicados. A resistência a antibióticos de bactérias patogênicas também foi verificada contra extratos de plantas de Ricinus communis, todos os patógenos bacterianos isolados foram resistentes, mas apenas E. coli foi inibida em 300µl dos extratos. A presença de bactérias patogênicas nas amostras de suco de maçã deveu-se à contaminação da água de esgoto na água potável, enquanto algumas dessas bactérias patogênicas vieram da árvore da maçã e outras de armazéns de frutas.

A introdução precoce de sucos pode influenciar desfechos antropométricos e consumo alimentar em idade pré-escolar? / Does early fruit juice introduction influence anthropometric outcomes and food consumption at preschool age?

Resumo Objetivou-se avaliar o impacto do consumo de sucos antes dos 6 meses de idade no Índice de Massa Corporal para Idade (IMC/I) e no consumo alimentar em pré-escolares. Estudo longitudinal com amostra composta por mães e seus filhos (n=103) entre 6 meses e 3 a 6 anos. Peso e estatura foram aferidos e convertidos para o escore z de IMC/I. Para avaliar o consumo alimentar, foi utilizado o Questionário de Frequência Alimentar Infantil. Testes de Qui-Quadrado e t de Student foram utilizados para comparação entre os grupos. Não houve diferença nos parâmetros antropométricos entre pré-escolares que receberam ou não suco antes dos seis meses. Aquelas que tiveram introdução de suco mais precoce (≤150 dias de vida) apresentaram consumo mais frequente (≥1x/dia) de suco artificial (63,8% versus 35,7%; p=0,028) e biscoito recheado (21,3% versus 14,3%; p=0,001) na idade pré-escolar. Crianças que receberam suco do tipo artificial antes dos 6 meses tiveram maior prevalência de consumo de refrigerante entre 1 e 4x/semana (69,2% versus 27,4%; p=0,014) e menor prevalência de consumo de achocolatado pelo menos 1x/dia (38,5% versus 69,4%; p=0,027). Sendo assim, crianças com introdução precoce de suco apresentaram maior consumo de alimentos doces e bebidas açucaradas em fase pré-escolar.

Abstract The aim of this study was to evaluate the impact of fruit juice consumption before 6 months of age on Body Mass Index-for-age (BMI-for-age) and food consumption in preschoolers. We conducted a longitudinal study with mothers and their children (n=103) at 6 months and 3-6 years. Weight and height were measured and converted into BMI-for-age z-scores. Food consumption was analyzed using the Food Frequency Questionnaire for Children. Groups were compared using the chi-squared and Student's t-tests. No differences in anthropometric measurements were found at preschool age between children who had been given fruit juice before 6 months and those who had not. Consumption of artificial juice (≥once/day) and sandwich cookies at preschool age was higher in children with early introduction of fruit juice (≤150 days of life) (63.8% versus 35.7%; p=0.028 and 21.3% versus 14.3%; p=0.001, respectively). The prevalence of the consumption of soda (1 to 4 times/week) and chocolate milk (at least once/day) was higher in children who had been given artificial juice before 6 months (69.2% versus 27.4%; p=0.014 and 38.5% versus 69.4%; p=0.027, respectively). It can be concluded that the consumption of sweet foods and sugary beverages was higher in children with early introduction of fruit juice.

Influência do Consumo de Suco de Laranja (Citrus Sinensis) na Remodelação Cardíaca de Ratos Submetidos a Infarto do Miocárdio / Influence of Consumption of Orange Juice (Citrus Sinensis) on Cardiac Remodeling of Rats Submitted to Myocardial Infarction

Resumo Fundamento O suco de laranja (SL) é rico em polifenóis com propriedades anti-inflamatórias e antioxidantes. Após o infarto do miocárdio (IM), mudanças complexas ocorrem na estrutura e na função cardíacas, processo conhecido como remodelação cardíaca (RC). O estresse oxidativo e a inflamação podem modular esse processo. Nossa hipótese foi a de que o consumo de SL atenua a RC após o IM. Objetivos Avaliar a influência do SL sobre a RC após IM pela análise de variáveis funcionais, morfológicas, de estresse oxidativo, de inflação, e de metabolismo energético. Métodos Um total de 242 ratos machos pesando entre 200 e 250g foram submetidos a um procedimento cirúrgico (ligação da artéria coronária ou cirurgia simulada). Sete dia após a cirurgia, os animais sobreviventes foram divididos para um dos quatro grupos: 1) SM, animais sham que receberam água e maltodextrina (n= 20); 2) SSL, animais sham que receberam SL (n= 20); 3) IM, animais infartados que receberam água e maltodextrina (n= 40); e 4) ISL, animais infartados que receberam SL (n = 40). A análise estatística foi realizada pelo teste de ANOVA com dois fatores com o teste de Holm-Sidak. Os resultados foram apresentados em média ± desvio padrão, e o nível de significância adotado foi de 5%. Resultados Três meses depois, o IM levou à hipertrofia do ventrículo esquerdo (VE), com disfunção sistólica e diastólica, e aumento nos mediadores inflamatórios e de estresse oxidativo. Os animais que consumiram SL apresentaram menor atividade da glutationa peroxidase e maior expressão da heme-oxigenase-1 (HO-1). Conclusão O SL atenuou a RC, e a HO-1 pode exercer um importante papel nesse processo.

Abstract Background Orange juice (OJ) is rich in polyphenols with anti-inflammatory and antioxidant properties. After myocardial infarction (MI), complex changes occur in cardiac structure and function, which is known as cardiac remodeling (CR). Oxidative stress and inflammation can modulate this process. We hypothesized that the consumption of OJ attenuates the CR after MI. Objectives To evaluate the influence of OJ on CR after MI by analysis of functional, morphological, oxidative stress, inflammation, and energy metabolism variables. Methods A total of 242 male rats weighing 200-250 g were submitted to a surgical procedure (coronary artery ligation or simulated surgery). Seven days after surgery, survivors were assigned to one of the four groups 1) SM, sham animals with water and maltodextrin (n= 20); 2) SOJ, sham animals with OJ (n= 20); 3) IM, infarcted animals with water and maltodextrin (n= 40); and 4) IOJ, infarcted animals with OJ (n = 40). Statistical analysis was performed by the two-way ANOVA supplemented by Holm-Sidak. Results are presented as mean ± standard deviation, the level of significance adopted was 5%. Results After 3 months, MI led to left ventricular (LV) hypertrophy, with systolic and diastolic dysfunction, and increased oxidative stress and inflammatory mediators. OJ intake reduced LV cavity and improved systolic and diastolic function. The OJ animals presented lower activity of glutathione peroxidase and higher expression of heme-oxygenase-1 (HO-1). Conclusion OJ attenuated CR in infarcted rats and HO-1 may be play an important role in this process.

Análise da adequação do termo detox nos rótulos de bebidas industrializadas à base de frutas e vegetais / Analysis of the adequacy of the term detox on the labels of industrialized drinks based on fruits and vegetables

Dietas populares, como as detox, surgem a todo o momento. Todavia, poucos dados científicos comprovam a eficácia e segurança destas dietas. Este trabalho avaliou rótulos de bebidas à base de frutas e vegetais e identificou as que se autodenominam detox. Tratou-se de estudo transversal descritivo, realizado de setembro a outubro/2016. O tamanho da porção na informação nutricional e as alegações encontradas nos rótulos foram analisados em relação às legislações vigentes. Composição nutricional, quantidade total de ingredientes e presença de aditivos alimentares e/ou outros ingredientes não usuais em preparações culinárias foram comparados entre as bebidas detox e as comuns. De 83 produtos analisados, 9,6% (n=8) se autodenominaram detox. A composição nutricional e o número médio de ingredientes não diferiram entre as bebidas detox e as comuns. Alegações nutricionais e de saúde estavam presentes em 77,1% (n=64) dos produtos e 53,0% (n=44) apresentaram alegações não previstas na RDC nº 54/2012. As informações mais frequentes foram quanto aos teores de micronutrientes, açúcares, sódio e fibras. O termo detox, apesar de não permitido, é encontrado neste tipo de produto no mercado brasileiro. Os resultados sugerem que o termo detox seja utilizado mais como estratégia de marketing do que como real alegação de propriedade nutricional. (AU)

Popular diets, such as detox, appear all the time. However, few scientific data prove the efficacy and safety of these diets. This work evaluated labels of drinks based on fruits and vegetables and identified those that call detox. This was a descriptive cross-sectional study, carried out from September to October/2016. The portion size in the nutrition information and the claims found on the labels were analyzed in relation to the current legislation. Nutritional composition, total amount of ingredients and presence of food additives and other unusual ingredients in culinary preparations were compared between detox and ordinary drinks. Of 83 products analyzed, 9.6% (n=8) called detox. The nutritional composition and the average number of ingredients did not differ between detox drinks and ordinary drinks. Nutritional and health claims were present in 77.1% (n=64) of the products and 53.0% (n=44) presented claims not provided for in RDC nº 54/2012. The most frequent information was about the levels of micronutrients, sugars, sodium and fibers. The term detox, although not allowed, is found in this type of product in the Brazilian market. The results suggest that the term detox is used more as a marketing strategy than as a real claim of nutritional property. (AU)

Análise de série temporal do consumo de bebidas açucaradas entre adultos no Brasil: 2007 a 2014 / Time-series analysis of the consumption of sweetened soft drinks among adults in Brazil: 2007 to 2014

Resumo O objetivo deste artigo é analisar a tendência temporal e os fatores associados ao consumo de refrigerante ou suco artificial entre adultos no Brasil. Estudo desenvolvido a partir de dados secundários do Sistema de Vigilância de Fatores de Risco e Proteção para Doenças Crônicas por Inquérito Telefônico, realizado com adultos brasileiros entre 2007-2014. Foi verificada a frequência e a intensidade do consumo (quantidade de copos ou latas por semana) de refrigerante ou suco artificial. Dados sociodemográficos e comportamentais foram as variáveis independentes. A tendência temporal do consumo anual foi avaliada por meio de Regressão Linear. Os fatores associados (idade, sexo, região, trabalho, escolaridade, hábito de assistir TV) ao consumo dessas bebidas foram investigados por Regressão de Poisson. Houve redução de 32,7% do consumo de refrigerante ou suco artificial entre 2007 e 2014. Os fatores associados ao maior consumo foram: sexo masculino (p = 0,000); faixa etária de 18-29 anos (p = 0,000); residência nas regiões centro-oeste, sudeste e sul (p = 0,000); menor escolaridade (p = 0,616); estar empregado (p = 0,007) e assistir TV mais de 3 horas por dia (p = 0,000). As análises descrevem uma tendência de queda no consumo de refrigerante ou suco artificial entre os adultos no Brasil de 2007 a 2014.

Abstract The scope of this article is to analyze the time-series trend and factors associated with the consumption of soft drinks or packaged fruit juices among adults in Brazil. It is a study based on secondary data from the System of Surveillance of Risk Factors and Protection for Chronic Diseases by Telephone Survey conducted among Brazilian adults between 2007 and 2014. The consumption frequency and intensity (number of cups or cans per week) of soda or packaged juice was checked. Socio-demographic and behavioral data were the independent variables. The time-series trend of annual consumption was evaluated by means of Linear Regression. The factors (age, sex, region, work, schooling and TV screen time) associated with the consumption of these beverages were investigated by Poisson regression. There was a 32.7% reduction in soft drink or packaged juice consumption between 2007 and 2014. Factors associated with higher consumption were: male sex (p = 0.000); 18-29 year-age-range (p = 0.000); residence in the central-west, southeast and southern regions (p = 0.000); lower schooling (p = 0.616); being employed (p = 0.007) and more than 3 hours of TV screen time per day (p = 0.000). The analyses describe a downward trend in the consumption of soda or packaged fruit juice among adults in Brazil from 2007 to 2014.

Potencial erosivo de jugos de frutas amazónicas / Erosive potential of juices of amazonian fruits

RESUMEN La erosión dental es la pérdida irreversible de la superficie del esmalte o dentina, debido a exposición frecuente de ácidos de origen no bacteriano. A pesar de que ciertos jugos de frutas presentan potencial erosivo in vitro, no existe una evidencia conclusiva al respecto. Objetivos: Evaluar in situ el potencial erosivo de jugos de frutas amazónicas sobre el esmalte dental. Material y métodos: Se evaluó in situ la microdureza superficial Vickers (MSV) del esmalte dental luego de la exposición a las bebidas Physalis Peruviana (aguaymanto), Averrhoa Carambola (carambola), Myrciaria Dubia (camu camu), Coca cola (control positivo) y agua destilada (control negativo). Participaron quince voluntarios (18 a 30 años) quienes utilizaron placas palatinas para reproducir el medio de almacenamiento. Se colocaron 6 fragmentos por placa. Las placas fueron expuestas a las diferentes bebidas 4 v/d (9:00 am, 11:00 am, 13:00 pm y 15:00 pm) durante 5 minutos por 5 días. Por cada ciclo erosivo se expuso a dos bebidas, inmediatamente los dispositivos palatinos fueron recolocados en la boca de los participantes. Hubo un periodo de washout de 8 días entre cada ciclo. Se calculó la diferencia de MSV antes y después de la exposición. El análisis de ANOVA/Tukey se utilizó para determinar las diferencias entre las bebidas en el programa SPSS 19, con un nivel de significancia de 0,5%. Resultados: Las cuatro bebidas evaluadas disminuyeron la MSV del esmalte (p<0,05) sin diferencia significativa entre ellas. Conclusiones: Los jugos a base de camu camu, aguaymanto y carambola son potencialmente erosivos.

SUMMARY Dental erosion is the irreversible loss of the surface of the enamel or dentin, due to frequent exposure of non- bacterial acids. Although certain fruit juices have erosive potential in vitro, there is no conclusive evidence in this regard. Objective: To evaluate in situ the erosive potential of Amazonian fruit juices on dental enamel. Material and methods: The Vickers surface microhardness (VMH) of dental enamel was evaluated in situ after exposure to drinks Physalis Peruviana (aguaymanto), Averrhoa Carambola (carambola), Myrciaria Dubia (camu camu), Coca cola (positive control) and distilled water (negative control). Fifteen volunteers (18 to 30 years) participated who wore palatal appliances to reproduce the storage medium. Each one containing 6 enamel blocks. The appliances were exposed to the different drinks 4 times a day (9:00 am, 11: 00 am, 13:00 pm and 15:00 pm) for 5 minutes during 5 days. For each erosive cycle, they were exposed to two drinks, immediately the palatal devices were repositioned in the participants' mouth. There was an 8-day washout period between each cycle. The difference in VMH before and after exposure was calculated. The ANOVA/Tukey analysis was used to determine the differences between beverages in the SPSS 19 program, with a significance level of 0.5%. Results: The four experimental groups decreased enamel VMH (p<0.05) without significant difference between them. Conclusions: The juices based on camu camu, aguaymanto and carambola are potentially erosive.

Nutritional profile of sugar-sweetened beverages destined to the youngsters marketed in Brazil and Portugal / Perfil nutricional de bebidas açucaradas destinadas ao público infantojuvenil comercializadas no Brasil e em Portugal

ABSTRACT Objective To analyze and compare the sugar content in sugar sweetened beverages in Brazil and Portugal, according to the Pan American Health Organization nutritional profile model. Methods One hundred six equivalent sugary beverages were selected in the markets of São Paulo, Brazil and Porto, Portugal. Nutritional information reported on the labels was reviewed, according to the Pan American Health Organization nutritional profile model, regarding the critical content of free sugars, salt, total saturated fats and trans fatty acids. Results The Brazilian nectars presented higher amounts of sugar and vitamin C compared to Portuguese nectars that contained more protein. The juices in Portugal contained a higher content of vitamin C and Portuguese dairy beverages had higher levels of protein and sodium, while the Brazilian ones had more total fats. According to the critical nutrients proposed by the Pan American Health Organization, Brazil had excess sodium in dairy beverages and soft drinks, while in Portugal excess sodium was found in nectars and dairy beverages. In both countries, excess free sugars were present in all beverages. Conclusion The amount of sugar in the beverages represents an important contribution of added sugar, especially in the Brazilian nectars. The identification of this component in sugary beverages is fundamental for the adoption of strategies of food education to control obesity.

RESUMO Objetivo Este estudo buscou analisar e comparar o teor de açúcar presente em bebidas prontas para consumo no Brasil e em Portugal, segundo o modelo de perfil nutricional da Organização Pan-Americana de Saúde. Métodos Selecionaram-se 106 bebidas açucaradas comercializadas nos mercados das cidades de São Paulo e do Porto. Analisaram-se informações nutricionais nos rótulos, conforme o modelo de perfil nutricional da Organização Pan-Americana de Saúde, quanto ao teor crítico de açúcares livres, sal, gorduras totais, saturados e ácidos graxos trans. Resultados Os néctares brasileiros apresentaram maior quantidade de açúcar e vitamina C; os portugueses evidenciaram maior valor proteico. Os sucos em Portugal continham maior teor de vitamina C, e as bebidas lácteas portuguesas demonstraram ter maior teor de proteína e sódio; já as brasileiras, maior quantidade de gorduras totais. De acordo com os nutrientes críticos propostos pela Organização Pan-Americana de Saúde, o Brasil possuiu excesso de sódio nas bebidas lácteas e refrigerantes, enquanto Portugal, nos néctares e nas bebidas lácteas. Em ambos os países, o excesso de açúcares livres esteve presente em todas as bebidas. Conclusão A quantidade de açúcar nas bebidas representa uma expressiva contribuição de açúcar adicionado, destacando-se os néctares brasileiros. A identificação desse componente em bebidas açucaradas é fundamental para a adoção de estratégias de educação alimentar contra a obesidade.

Microencapsulation of Lactobacillus plantarum 299v and its storage in kuini juice

Aims@#Probiotics are living microorganism, when administrated in sufficient quantity can exert beneficial effect to the host. This study focused on the microencapsulation by co-extrusion to increase the viability of Lactobacillus plantarum 299v (Lp299v) in gastrointestinal conditions, and its storage stability in kuini juice at refrigerated (4 °C) and ambient temperature (25 °C). @*Methodology and results@#Lp99v was encapsulated with 1.5% w/v sodium alginate and chitosan coating (0.1% w/v) and yielded a microencapsulation efficiency of 97.71%. The Lp299v microbeads produced were spherical in shape and exhibited a mean microbeads size of 618.75 ± 25.85 µm. Acid and bile tolerance of both free and encapsulated Lp299v were tested in simulated gastric juice (SGJ) for 2 h and in simulated intestinal juice (SIJ) for 4 h, respectively. The encapsulated Lp299v maintained above 108 CFU/mL after exposure to artificial gastrointestinal juice, whereas a significant loss of viability was observed in the free cells. The storage stability of encapsulated Lp299v in kuini juice was determined during 4 weeks of storage at 4 °C and 25 °C. Results showed that encapsulated Lp299v was capable to remain viable (107 CFU/mL) for at least 4 weeks in a refrigerated condition. However, free Lp299v did not survived under both refrigerated and ambient temperature as the storage period extended. @*Conclusion, significance and impact of study@#Lp299v entrapped in chitosan-coated alginate microbeads produced by co-extrusion method is able to enhance the viability of Lp299v above the minimum recommended level in harsh environment (gastrointestinal conditions and low pH of kuini juice).

Indian Academy of Pediatrics Guidelines on the Fast and Junk Foods, Sugar Sweetened Beverages, Fruit Juices, and Energy Drinks

Justification: In view of easy availability and increasing trend of consumption of fast foods and sugar sweetened beverages (fruit juicesand drinks, carbonated drinks, energy drinks) in Indian children, and their association with increasing obesity and related non-communicable diseases, there is a need to develop guidelines related to consumption of foods and drinks that have the potential toincrease this problem in children and adolescents. Objectives: To review the evidence and formulate consensus statements related toterminology, magnitude of problem and possible ill effects of junk foods, fast foods, sugar-sweetened beverages and carbonated drinks;and to formulate recommendations for limiting consumption of these foods and beverages in Indian children and adolescents. Process:A National Consultative group constituted by the Nutrition Chapter of the Indian Academy of Pediatrics (IAP), consisting of variousstakeholders in private and public sector, reviewed the literature and existing guidelines and policy regulations. Detailed review ofliterature was circulated to the members, and the Group met on 11th March 2019 at New Delhi for a day-long deliberation on framing theguidelines. The consensus statements and recommendations formulated by the Group were circulated to the participants and aconsensus document was finalized. Conclusions: The Group suggests a new acronym ‘JUNCS’ foods, to cover a wide variety ofconcepts related to unhealthy foods (Junk foods, Ultra-processed foods, Nutritionally inappropriate foods, Caffeinated/colored/carbonated foods/beverages, and Sugar-sweetened beverages). The Group concludes that consumption of these foods and beveragesis associated with higher free sugar and energy intake; and is associated with higher body mass index (and possibly with adversecardiometabolic consequences) in children and adolescents. Intake of caffeinated drinks may be associated with cardiac and sleepdisturbances. The Group recommends avoiding consumption of the JUNCS by all children and adolescents as far as possible and limittheir consumption to not more than one serving per week. The Group recommends intake of regional and seasonal whole fruits over fruitjuices in children and adolescents, and advises no fruit juices/drinks to infants and young children (age <2 y), whereas for children aged 2-5 y and >5-18 y, their intake should be limited to 125 mL/day and 250 mL/day, respectively. The Group recommends that caffeinatedenergy drinks should not be consumed by children and adolescents. The Group supports recommendations of ban on sale of JUNCSfoods in school canteens and in near vicinity, and suggests efforts to ensure availability and affordability of healthy snacks and foods. TheGroup supports traffic light coding of food available in school canteens and recommends legal ban of screen/print/digital advertisementsof all the JUNCS foods for channels/magazines/websites/social media catering to children and adolescents. The Group further suggestscommunication, marketing and policy/taxation strategies to promote consumption of healthy foods, and limit availability and consumptionof the JUNCS foods

Antioxidant and Vasodilatory Action of Grape Juices Produced in Different Regions of Brazil

Grapes and its derivatives (wines and juices) are rich in polyphenols that have high antioxidant and vasodilator capacity. These biological activities may vary in the juices marketed and produced in different regions of Brazil. Objectives: To determine the antioxidant and vasorelaxant effects of grape juice samples produced in different regions of Brazil. Methods: The content of phenolic compounds and antioxidant capacity were evaluated by the methods of Folin-Ciocalteau, DPPH, ABTS and a new electroanalytical approach (differential pulse voltammetry - DPV). Vasodilator effects were analyzed in isolated aorta from rats in an organ bath. Results: The samples from RJ and SP presented respectively the higher and lower phenolic content and also antioxidant capacity by the methods used (ABTS and DPPH). The results of the electrochemical index corroborate to the other tests, with the best results to RJ (21.69 ± 3.15 µA/V) and worse to the SP sample (11.30 ± 0.52 µA/V). In the vascular reactivity studies, the relaxation induced by each sample presented more distinct differences, following the order: RJ (87.9 ± 4.8%) > RS1 (71.6 ± 8.6%) > GO (56.2 ± 7.2%) > SP (39.9 ± 7.8%) > PR (39.4 ± 9.5%) > RS2 (19.5 ± 6.2%). Inhibition of endothelial NO practically abolished (p < 0.001) the relaxation for all samples, except one. Conclusion: The phenolic content and antioxidant capacity vary greatly among samples. The results obtained for the order of antioxidant activity were: RJ > RS1 > GO > RS2 > PR > SP. The juices were able to induce vascular relaxation at quite varied levels, and the RJ sample the most effective. The L-NAME practically blocked all samples except one (RS2)

Consumo de bebidas açucaradas e fatores associados em adultos / Consumption of sweetened beverages and associated factors in adults

Resumo O objetivo deste estudo foi avaliar o consumo regular de bebidas açucaradas e sua associação com aspectos sociodemográficos e comportamentais de adultos de uma cidade do sudoeste brasileiro. Trata-se de um estudo de base populacional com 1226 adultos de 20 a 59 anos, residentes na zona urbana do município de Viçosa-MG. Foi aplicado um questionário estruturado para medir as condições sociodemográficas e comportamentais. O consumo de bebidas açucaradas foi classificado como regular quando a frequência de ingestão foi igual ou superior a cinco vezes por semana. O consumo regular de bebidas açucaradas foi superior em homens e mulheres com idade entre 20 e 29 anos (p < 0,01). Homens com hábito de realizar refeições em frente à televisão (p = 0,03) e mulheres insatisfeitas com seu peso (p = 0,03) apresentaram maior consumo regular de bebidas açucaradas. Conclui-se que os indivíduos de maior idade apresentaram menor consumo regular de bebidas açucaradas e este consumo esteve associado a comportamentos obesogênicos, como o hábito em realizar refeições em frente à televisão, e em indivíduos insatisfeitos com o peso corporal.

Abstract The aim of this study was to evaluate the regular consumption of sweetened beverages and its association with sociodemographic and behavioral aspects in a city in the southwest of Brazil. It involves is a population-based study of 1,226 adults aged 20 to 59 living in the urban area of the municipality of Viçosa in the state of Minas Gerais. A structured questionnaire to measure sociodemographic and behavioral conditions was applied. The consumption of sweetened beverages was considered regular when the frequency of consumption was five or more times a week. Regular consumption of sweetened beverages was higher in men and women aged between 20 and 29 years (p < 0.01). Men with the habit of eating meals in front of the television (p = 0.03) and women dissatisfied with their weight (p = 0.03) consumed greater regular amounts of sweetened beverages. The conclusion reached is that older individuals showed less regular consumption of sweetened beverages and that the consumption of same was associated with obesogenic behaviors, such as the habit of eating meals in front of the television and among individuals dissatisfied with their body weight.

Posicionamiento sobre los impuestos a alimentos no básicos densamente energéticos y bebidas azucaradas / Position paper on taxes to non-basic energy-dense foods and sugar-sweetened beverages

Resumen: En 2014, el gobierno mexicano implementó una política fiscal para disminuir el consumo de bebidas azucaradas y alimentos no básicos con alta densidad energética. Este documento resume y analiza los estudios que han evaluado el impacto observado y esperado de los impuestos a alimentos y bebidas no saludables en México. La implementación de impuestos ha logrado disminuir las compras de bebidas azucaradas en 7.6% y alimentos no básicos con alta densidad energética en 7.4%. La reducción de consumo de bebidas azucaradas podría reducir la obesidad en 2.5%, prevenir 20 000 casos de enfermedad cardiovascular y 189 000 casos de diabetes al año 2022, así como prevenir 2 000 muertes. Además, se espera que estos impuestos ayuden a reducir en 1 173 millones de dólares los costos de atención a la salud. Los impuestos a alimentos no saludables deben fortalecerse y permanecer como parte integral de la estrategia nacional dirigida a reducir la obesidad y las enfermedades crónicas en México.

Abstract: In 2014 the Mexican government implemented taxes to reduce the consumption of sugar-sweetened beverages and nonessential energy-dense foods. In this manuscript, we analyze the scientific evidence linking the consumption of these products to chronic diseases, and summarize the studies that have evaluated the observed and expected impact of food taxes in Mexico. The implementation of taxes to unhealthy foods has reduced purchases of sugar-sweetened beverages in 7.6% and nonessential energy-dense foods in 7.4%. A reduction in consumption could decrease obesity prevalence by 2.5%; prevent 20 000 cardiovascular disease events; 189 000 diabetes cases; and 20 000 cardiovascular deaths. Additionally, this impact could save nearly 1 173 million dollars from healthcare costs. Taxes to unhealthy foods should be strengthened up and remain as an integral part of the national strategy to reduce obesity and chronic diseases in Mexico.

Qualidade microbiológica de refrescos armazenados em garrafas PET e refresqueiras comercializados em um município do Recôncavo da Bahia

Este trabalho teve por objetivo avaliar as características higienicossanitárias dos refrescos armazenados em garrafas tipo PET e em refresqueiras comercializados em estabelecimentos de um município do Recôncavo da Bahia. Foram analisadas, quanto à presença de bolores, leveduras e coliformes termotolerantes, 10 amostras de refrescos, acondicionadas em garrafas PET e refresqueiras. Das cinco amostras analisadas, duas (40%) armazenadas em garrafas PET, e uma (20%) armazenada em refresqueiras, apresentou-se em desacordo com os padrões estabelecidos pela legislação vigente. Na análise de bolores e leveduras, encontrou-se elevado crescimento destes micro-organismos em quatro amostras (80%) armazenadas em garrafas PET e em cinco amostras (100%) armazenadas em refresqueiras. As informações obtidas nessa pesquisa demonstraram que os refrescos coletados estavam em condições higienicossanitárias insatisfatórias, podendo representar causas de infecções de origem alimentar aos consumidores.

The objective of this study was to evaluate the hygienic-sanitary characteristics of refreshment stored in PET bottles and in refreshments marketed in establishments in a municipality in the Recôncavo da Bahia. For the presence of molds, yeasts and thermotolerant coliforms, 10 samples of refreshment, packed in PET bottles and coolers, were analyzed. Of the five samples analyzed, two (40%) stored in PET bottles, and one (20%) stored in refrigerators, were in disagreement with the standards established by current legislation. When analyzing molds and yeasts, a high growth of these microorganisms was found in four samples (80%) stored in PET bottles and in five samples (J 00%) stored in coolants. The information obtained in this research showed that the refreshments collected were in unsatisfactory hygienic-sanitary conditions, and may represent causes of food-borne infections to consumers.

Qualidade microbiológica de refrescos armazenados em garrafas pet e refresqueiras comercializados em um munícipio do recôncavo da Bahia / Microbiological quality of soft drinks stored in pet bottles marketed in a municipality of the Bahia state

Este trabalho teve por objetivo avaliar as características higienicossanitárias dos refrescos armazenados em garrafas tipo PET e em refresqueiras comercializados em estabelecimentos de um município do Recôncavo da Bahia. Foram analisadas, quanto à presença de bolores, leveduras e coliformes termotolerantes, 10 amostras de refrescos, acondicionadas em garrafas PET e refresqueiras. Das cinco amostras analisadas, duas (40%) armazenadas em garrafas PET, e uma (20%) armazenada em refresqueiras, apresentou-se em desacordo com os padrões estabelecidos pela legislação vigente. Na análise de bolores e leveduras, encontrou-se elevado crescimento destes micro- -organismos em quatro amostras (80%) armazenadas em garrafas PET e em cinco amostras (100%) armazenadas em refresqueiras. As informações obtidas nessa pesquisa demonstraram que os refrescos coletados estavam em condições higienicossanitárias insatisfatórias, podendo representar causas de infecções de origem alimentar aos consumidores.(AU)

The objective of this study was to evaluate the hygienic-sanitary characteristics of refreshment stored in PET bottles and in refreshments marketed in establishments in a municipality in the Recôncavo da Bahia. For the presence of molds, yeasts and thermotolerant coliforms, 10 samples of refreshment, packed in PET bottles and coolers, were analyzed. Of the five samples analyzed, two (40%) stored in PET bottles, and one (20%) stored in refrigerators, were in disagreement with the standards established by current legislation. When analyzing molds and yeasts, a high growth of these microorganisms was found in four samples (80%) stored in PET bottles and in five samples (100%) stored in coolants. The information obtained in this research showed that the refreshments collected were in unsatisfactory hygienic-sanitary conditions, and may represent causes of food-borne infections to consumers.

Potencial erosivo de jugos naturales, jugos industrializados y gaseosas: Revisión de Literatura / Erosive potential of natural juices, industrialized and carbonated beverages: Literature review

El estudio de las lesiones dentales de origen no carioso ha ganado un gran terreno en el último siglo, siendo cada vez más frecuente encontrar lesiones como erosión, abfracción, atrición, y abrasión. El consumo cotidiano de jugos naturales, jugos industrializados o de gaseosas postula como un factor preponderante en la etiología de la erosión dental. Existe literatura muy amplia que aborda supotencial erosivo, características de estas bebidas, la frecuencia de ingesta, edad de la persona, entre otros. El objetivo de esta revisión de literatura es presentar evi- dencia científica sobre el potencial erosivo en la dentición humana de los jugos naturales, jugos industrializados y gaseosas.

The study of dental lesions of non-carious origin has gained a lot of ground in the last century, being increasingly frequent to find injuries such as erosion, abfraction, attrition, and abrasion. The daily consumption of natural juices, industrialized juices orcarbonated juices postulates as a preponderant factor in the etiology of dental erosion. There is a very broad literature that addresses its erosive potential, characteristics of these drinks, the frequency of intake, age of the person, among others. The objective of this literature review is to present scien- tific evidence on the erosive potential in human dentition of natural juices, industrialized and carbonated juices.

Milk Reverts the Effects of an Enamel Erosive but Healthy Diet

Objective: To determine the erosive effects of popular fruits and vegetables on enamel, and the potential counter effects of milks. Material and Methods: Ten popular fruits and vegetables were individually blended in a juicer to form a fruit or vegetable juice. The labial surfaces (three for each treatment group) were then immersed for thirty minutes in a fresh juice of one of ten products: strawberry, spinach, pineapple, grapefruit, green pepper, pickle, orange, apple, tomato, and carrot. In a second experiment, human enamel was treated with Coca-Cola for 30 minutes, followed by a variety of milks: Skim, 2%, Whole, Chocolate, Lactaid, Almond, and Silk Soy. Three teeth were used for each treatments and enamel microhardness of each sample was measured at baseline, after erosive exposure, and after the 30-minute variable treatment. Results: All fruits and vegetables showed differences between the pre- and posttreatment microhardness values. For the second experiment, tested milks result in significant increases (p<0.05) in enamel microhardness. Paired-t test was used for all comparisons. A thirty-minute exposure to the juices of all fruits and vegetables examined in the study significantly changed the microhardness of enamel, with grapefruit and pickle having potentially the most erosive effect on enamel. Diets that are high in fruits and vegetables can predispose to enamel erosion. For the second experiment, filtered water, ACT fluoride rinse, and varying milks all result in remineralization of enamel. Conclusion: Eroded enamel exposed to milk demonstrated a significant greater gain of enamel microhardness as compared to filtered water.

Evaluation of Total Phenolic Content, Antioxidant Activities and Sugar Content of Fresh Mixed Fruit and Vegetables Juices / Jurnal Sains Kesihatan Malaysia

The notion of fruit and vegetables (FV) in preventing chronic diseases has long been discerned. To meet the recommended FV intake, FV juices have emerged as a convenient and healthy choice. Mixed fruit and vegetable juices (MFVJ) cater the blend of desirable flavor and taste of consumers. This study was carried out to determine the total phenolic content (TPC), antioxidant activities and sugar content of selected MFVJ. Folin-Ciocalteu assay was employed to quantify TPC, whereas DPPH and FRAP assays were used to evaluate the antioxidant activities of MFVJ. The sugar content was determined using phenol-sulfuric acid method. MFVJ extracted from bitter gourd, green apple and orange (BGO) had the highest TPC content (76.4 ± 4.2 mg GAE/100 ml). Celery and green apple (CG) juice showed the highest DPPH value (522.3 ± 7.6 mg TE/100 ml) whereas carrot and starfruit (AS) juice have the highest FRAP value (419.6 ± 21.6 mg TE/100 ml). The sugar content of MFVJ was within the range of 5.7-13.3 g/100 ml. MFVJ can be considered as healthy beverages with considerable amounts of phenolic compounds and low sugar content. This study provides some useful reference for consumers who consume juices with combinations of FV. Future studies need to discover more combinations of FV juices, providing more data pertaining to MFVJ. Identification of individual phenolic compounds should also be part of future research using various instrumental analyses.

Functional restructured meat: applications of ingredients derived from plants / Reestructurados cárnicos funcionales: aplicación de ingredientes derivados de plantas

Background: Meat is an important source of nutrients. However, in recent years their consumption is associated with chronic-degenerative diseases giving it the perception of "unhealthy food" Given that meat is an affordable source of quality protein; its improvement entails a huge challenge for the industry and science. Methods: The search and structured review of the literature in the last ten years in the scientific databases of articles related to the elaboration of restructured meat products with functional ingredients derived from plants. Objective: This work presents a general overview, as well as the most representative studies on the elaboration of restructured meat with ingredients from plants considered functional. Conclusions: The present review is intended to emphasize the use of plant natural ingredients in the elaboration of functional restructured meat products as an alternative for consumers allowing the inclusion of functional compounds beneficial to human health in their daily diet

Antecedentes: La carne es una fuente importante de nutrientes. Sin embargo, en los últimos años su consumo se asocia a enfermedades crónico-degenerativas dando la percepción de alimento poco saludable. Dado a que es una fuente accesible de proteína de calidad, su mejoramiento implica un enorme desafío para la industria y la ciencia de la carne. Método: La revisión estructurada de diversos artículos de investigación encontrados en bases de datos científicas, durante los últimos 10 años, relacionados a la elaboración de reestructurados cárnicos con derivados de plantas considerados funcionales. Objetivo: Este trabajo presenta una revisión general, de los estudios más representativos sobre la elaboración reestructurados cárnicos elaborados con derivados de plantas considerados como funcionales. Conclusión: La elaboración de productos reestructurados cárnicos funcionales con la utilización de derivados vegetales, puede considerarse una alternativa para los consumidores a fin de incluir compuestos funcionales beneficiosos para la salud humana en la dieta diaria

Effects of corn oil on the volatile fatty acids in horses with induced gastric ulcers / Efecto del aceite de maíz sobre los ácidos grasos volátiles en caballos con úlceras gástricas inducidas

ABSTRACT Objetive. To determine the influence of corn oil on the volatile fatty acids (VFA) concentrations in the gastric juice in horses with phenylbutazone (PBZ) induced gastric ulcers and Correlate the gastroscopic findings with the VFA concentrations. Materials and methods. 15 horses were allotted in 3 groups. Group I (control) received placebo during first 6 days (induction period) and was treated with sucralfate for 2 weeks (treatment period). Groups II and III received PBZ during the induction phase. After 6 days, horses from group II received 70 mL of corn oil /100 kg of body weight/ po, twice a day, for 2 weeks and horses from group III received 90 mL of corn oil/100 kg of body weight/ po, twice a day, for 2 weeks. All horses were examined by gastroscopy at days 0, 7 and 21. The lesions were recorded and classified according to the number and severity. Samples from gastric fluid were taken to measure the concentrations of the acetic, propionic, butyric and lactic acids. Results. Both PBZ protocols produced lesions in the both non-glandular and glandular areas of the stomach. All the treatments produced healing of the injured mucosa glandular. Neither of the two corn oil treatments affected healing of the gastric ulcers located in the non-glandular area. Conclusions. The concentrations of acetic and butyric acids were highest in the gastric juice. The corn oil and sucralfate did not lead to differences in the concentration of acetic acid and butyric acid.

RESUMEN Objetivo. Determinar el efecto del aceite de maíz sobre la concentración de los ácidos grasos volátiles (AGV) en el jugo gástrico de caballos con úlceras gástricas inducidas con fenilbutazona (FBZ) y correlacionar los hallazgos gastroscópicos con las concentraciones de AGV. Materiales y métodos. 15 caballos fueron asignados en 3 grupos. El grupo I (control) recibió placebo durante los primeros 6 días (período de inducción) y fue tratado con sucralfato durante 2 semanas (período de tratamiento). Los grupos II y III recibieron FBZ en la fase de inducción. Después de 6 días, los caballos del grupo II recibieron 70 ml de aceite de maíz /100 kg de peso corporal / vo, dos veces al día, durante 2 semanas y los caballos del grupo III recibieron 90 ml de aceite de maíz / 100 kg de peso corporal / vo, dos veces al día, durante 2 semanas. Todos los caballos fueron examinados por gastroscopia en el día 0, 7 y 21. Las lesiones fueron registradas y clasificadas según el número y severidad. Muestras de fluido gástrico se utilizaron para medir las concentraciones de los ácidos acético, propiónico, butírico y láctico. Resultados. Ambos protocolos de FBZ ocasionaron lesiones en las áreas aglandular y glandular del estómago. Todos los tratamientos reestablecieron el epitelio ulcerado de la mucosa glandular. Ninguno de los dos tratamientos a base de aceite de maíz reestablecieron el epitelio ulcerado de la zona aglandular del estómago. Conclusiones. Las concentraciones de los ácidos acético y butírico fueron más altas en el jugo gástrico. El aceite de maíz y el sucralfato no afectaron la concentración de ácido acético y ácido butírico.